《植物生理学报》 2015, 51(1): 93-96

低温下六个苹果新品种抗性生理指标的比较

闫忠业, 吕天星, 王冬梅, 刘志, 伊凯^*

辽宁省果树科学研究所, 辽宁熊岳115009

通信作者：伊；E-mail: lnyikai@163.com；Tel: 0417-7033992

摘　要：

以6个苹果新品种为试材, 在–45~–20 ℃之间设置6个温度梯度处理, 测定一年生休眠枝条的丙二醛(MDA)含量、过氧化物酶(POD)活性以及脯氨酸含量, 对6个苹果品种抗寒性进行评价。结果表明: 低温处理后, ‘望山红’的MDA含量一直较高, ‘七月鲜’的MDA含量较低且稳定; ‘七月鲜’的POD活性最高, 但各品种间差异不显著; ‘岳阳红’的脯氨酸含量最高, 显著高于其他品种, 其他品种间差异不显著, ‘七月鲜’的脯氨酸含量较稳定。可见, ‘七月鲜’的抗寒力最强, ‘望山红’较弱, 其他品种介于两者之间。抗寒品种表现为枝条中MDA含量低且较稳定, POD活性高, 脯氨酸含量稳定。

关键词：苹果; 抗寒性; 丙二醛; 过氧化物酶; 脯氨酸

收稿：2014-11-12   修定：2015-01-05

资助：现代农业产业技术体系建设专项资金(CARS-28)、辽宁省博士启动基金项目(20071061)、辽宁省科技攻关项目(2011204001)。

Comparison of Resistant Physiological Index among Six New Apple Cultivars after Low Temperature Treatment

YAN Zhong-Ye, LÜ Tian-Xing, WANG Dong-Mei, LIU Zhi, YI Kai^*

Liaoning Province Institute of Pomology, Xiongyue, Liaoning 115009, China

Corresponding author: YI Kai; E-mail: lnyikai@163.com; Tel: 0417-7033992

Abstract:

In order to evaluate cold tolerance of dormant shoots from six apple cultivars, we measured the contents of malondialdehyde (MDA) and proline, and the activity of peroxidase (POD) after the shoots were treated at six different temperatures from –45 ℃ to –20 ℃. It was found that MDA content was always high in ‘Wangshanhong’ shoots, and was always low and stable in ‘Qiyuexian’ after low temperature treatment. POD activity in ‘Qiyuexian’ shoots was highest, but was not significantly different from those in other cultivars. Proline content in ‘Yueyanghong’ shoots was highest and significantly higher than others, while there was no difference among the others. Proline level in ‘Qiyuexian’ shoots was stable. The results indicate that cold tolerance of ‘Qiyuexian’ is strongest and of ‘Wangshanhong’ is weakest, and of other cultivars is intermediate. The apple cultivars with strong cold tolerance show low and stable level of MDA, stable level of proline and high activity of POD in shoots.

Key words: apple; cold resistance; MDA; POD; proline